Androgen receptor mRNA expression in Xenopus laevis CNS: sexual dimorphism and regulation in laryngeal motor nucleus

J Neurobiol. 1996 Aug;30(4):556-68. doi: 10.1002/(SICI)1097-4695(199608)30:4<556::AID-NEU10>3.0.CO;2-D.

Abstract

Using Northern analysis, in situ hybridization, and nuclease protection assays, the expression and regulation of androgen receptor messenger RNA (AR mRNA) was examined in the CNS of juvenile Xenopus laevis. Only one of the AR mRNA isoforms expressed in X. laevis is transcribed in the CNS as shown by Northern blot analysis. Nuclease protection assays demonstrate that the expression of AR mRNA is higher in the brain stem than in the telencephalon and diencephalon. Although expression of AR mRNA is widespread throughout the CNS, cells of cranial nerve nucleus IX-X (N.IX-X) and spinal cord display the highest in situ hybridization signals in their cytoplasm. Double labeling using horseradish peroxidase and digoxigenin labeled AR probes reveals that laryngeal and anterior spinal cord motor neurons express AR mRNA. More cells express AR mRNA in N.IX-X of males than of females. The number of AR expressing cells in N. IX-X decreases following gonadectomy in both sexes, and dihydrotestosterone (DHT) treatment for 1 month reverses this effect. Increased expression of AR mRNA in the brain of DHT treated animals is also apparent in nuclease protection assays. Sex differences in number of AR expressing cells and hormone regulation of AR mRNA expression in motor nuclei may influence neuromuscular systems devoted to sexually differentiated behaviors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Castration
  • Central Nervous System / cytology
  • Central Nervous System / metabolism*
  • Central Nervous System / physiology*
  • Dihydrotestosterone / pharmacology
  • Female
  • Gene Expression Regulation
  • Larynx / physiology*
  • Male
  • Motor Neurons / metabolism
  • RNA, Messenger / metabolism*
  • Receptors, Androgen / genetics*
  • Sex Characteristics*
  • Tissue Distribution
  • Xenopus laevis / metabolism*

Substances

  • RNA, Messenger
  • Receptors, Androgen
  • Dihydrotestosterone