Vibrio cholerae hemagglutinin/protease (HA/protease) causes morphological changes in cultured epithelial cells and perturbs their paracellular barrier function

Microb Pathog. 1996 Aug;21(2):111-23. doi: 10.1006/mpat.1996.0047.


In this report, we describe the cytotoxic activity of the cholera hemagglutinin/protease (HA/protease). A concentrated protein sample from the 37 degrees C overnight culture supernatant of CVD110, a delta ctxA, delta zot, delta Ace and hlyA::(ctxB mer) mutant of El Tor biotype Ogawa serotype strain E7946 caused morphological changes in cultured MDCK-I epithelial cells and altered their arrangement of filamentous actin (F-actin) and Zonula occludens-associated protein ZO-1. The drastic morphological changes can be inhibited by Zincov, a specific bacterial metalloprotease inhibitor. The cytotoxic fractions of the sample after FPLC gelfiltration fractionation showed two visible protein bands with molecular weights of approximately 34- and 32 kDa. Microsequencing of these two proteins revealed that they were the cholera HA/protease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / drug effects
  • Animals
  • Biological Transport / drug effects
  • Cell Line
  • Cholera / etiology
  • Dogs
  • Electric Impedance
  • Epithelial Cells
  • Epithelium / drug effects
  • Hemagglutinins / toxicity*
  • Kidney / cytology
  • Membrane Proteins / drug effects
  • Metalloendopeptidases / toxicity*
  • Permeability / drug effects
  • Phosphoproteins / drug effects
  • Vibrio cholerae* / pathogenicity
  • Zonula Occludens-1 Protein


  • Actins
  • Hemagglutinins
  • Membrane Proteins
  • Phosphoproteins
  • Zonula Occludens-1 Protein
  • Metalloendopeptidases
  • hemagglutinin-protease