Differential expression of 70-kDa heat shock-protein in human oral tumorigenesis

Int J Cancer. 1995 Dec 11;63(6):774-9. doi: 10.1002/ijc.2910630604.

Abstract

To understand the biological events underlying the multistep process of oral tumorigenesis we have studied the expression of 70-kDa heat-shock protein, HSP70, in human normal, pre-malignant and malignant oral tissues. Expression of HSP70 was assessed in oral-tissue specimens using a mouse monoclonal antibody against HSP70 by immunostaining and immunoblotting analysis. Strong nuclear and cytoplasmic HSP70 immunostaining was observed in oral squamous-cell carcinomas (30/38). Mild to moderate HSP70 expression was observed in oral dysplastic lesions (19/30) and basal low level of HSP70 was observed in normal oral tissues. The results were corroborated by immunoblotting analysis. The wide variation in HSP70 expression in normal, pre-malignant and malignant oral lesions suggests that it is differentially expressed during oral carcinogenesis and may be implicated in tumor development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Squamous Cell / metabolism*
  • Female
  • HSP70 Heat-Shock Proteins / analysis*
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mouth Neoplasms / metabolism*

Substances

  • HSP70 Heat-Shock Proteins