Pre- and postsynaptic localization of a metabotropic glutamate receptor, mGluR2, in the rat brain: an immunohistochemical study with a monoclonal antibody

Neurosci Lett. 1996 Jan 5;202(3):197-200. doi: 10.1016/0304-3940(95)12248-6.


A monoclonal antibody against a metabotropic glutamate receptor, mGluR2, was produced by using a glutathione S-transferase (GST) fusion protein containing an N-terminal sequence of rat mGluR2. Intense mGluR2-like immunoreactivity (mGluR2-LI) was seen mainly in neuropil of the cerebral cortical regions, hippocampus, olfactory bulb, some diencephalic nuclei, dorsal cochlear nucleus and cerebellar cortex. In the cerebellar cortex, mGluR2-LI was seen only in Golgi cells. In Ammon's horn, mGluR2-LI was marked in the stratum lucidum of CA3 and the stratum lacunosum-moleculare of CA1-CA3, but not detected in the stratum pyramidale. The results indicate that mGluR2 is located not only presynaptically but also postsynaptically.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Brain / anatomy & histology*
  • Brain Chemistry / physiology*
  • Immunohistochemistry
  • Male
  • Membrane Potentials / physiology
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Rats, Wistar
  • Receptors, Metabotropic Glutamate / immunology
  • Receptors, Metabotropic Glutamate / metabolism*
  • Receptors, Presynaptic / metabolism*
  • Recombinant Fusion Proteins / immunology
  • Synapses / metabolism*


  • Antibodies, Monoclonal
  • Receptors, Metabotropic Glutamate
  • Receptors, Presynaptic
  • Recombinant Fusion Proteins