Inhibition of glycolysis in boar spermatozoa by alpha-chlorohydrin phosphate appears to be mediated by phosphatase activity

Reprod Fertil Dev. 1995;7(5):1089-94. doi: 10.1071/rd9951089.

Abstract

(R,S)-alpha-chlorohydrin-1-phosphate, previously shown to have no anti-glycolytic activity on mature boar sperm in vitro, is a substrate for acid and/or neutral phosphatase(s) that are associated with washed sperm. The high phosphatase activity hydrolyses the ester to alpha-chlorohydrin which undergoes oxidation to (S)-3-chlorolactaldehyde, a specific inhibitor of sperm glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, thereby exhibiting an anti-glycolytic action.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme Inhibitors / pharmacology*
  • Fructose / metabolism
  • Glyceraldehyde-3-Phosphate Dehydrogenases / antagonists & inhibitors
  • Glycolysis / drug effects*
  • Hydrogen-Ion Concentration
  • Hydrolysis
  • Male
  • Oxidation-Reduction
  • Phosphoric Monoester Hydrolases / metabolism*
  • Spermatozoa / drug effects
  • Spermatozoa / metabolism*
  • Swine*
  • Triose-Phosphate Isomerase / antagonists & inhibitors
  • alpha-Chlorohydrin / analogs & derivatives*
  • alpha-Chlorohydrin / metabolism
  • alpha-Chlorohydrin / pharmacology

Substances

  • Enzyme Inhibitors
  • alpha-chlorohydrin-1-phosphate
  • Fructose
  • alpha-Chlorohydrin
  • Glyceraldehyde-3-Phosphate Dehydrogenases
  • Phosphoric Monoester Hydrolases
  • Triose-Phosphate Isomerase