Abstract
(R,S)-alpha-chlorohydrin-1-phosphate, previously shown to have no anti-glycolytic activity on mature boar sperm in vitro, is a substrate for acid and/or neutral phosphatase(s) that are associated with washed sperm. The high phosphatase activity hydrolyses the ester to alpha-chlorohydrin which undergoes oxidation to (S)-3-chlorolactaldehyde, a specific inhibitor of sperm glyceraldehyde-3-phosphate dehydrogenase and triosephosphate isomerase, thereby exhibiting an anti-glycolytic action.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Enzyme Inhibitors / pharmacology*
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Fructose / metabolism
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Glyceraldehyde-3-Phosphate Dehydrogenases / antagonists & inhibitors
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Glycolysis / drug effects*
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Hydrogen-Ion Concentration
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Hydrolysis
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Male
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Oxidation-Reduction
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Phosphoric Monoester Hydrolases / metabolism*
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Spermatozoa / drug effects
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Spermatozoa / metabolism*
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Swine*
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Triose-Phosphate Isomerase / antagonists & inhibitors
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alpha-Chlorohydrin / analogs & derivatives*
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alpha-Chlorohydrin / metabolism
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alpha-Chlorohydrin / pharmacology
Substances
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Enzyme Inhibitors
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alpha-chlorohydrin-1-phosphate
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Fructose
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alpha-Chlorohydrin
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Glyceraldehyde-3-Phosphate Dehydrogenases
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Phosphoric Monoester Hydrolases
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Triose-Phosphate Isomerase