Analysis of anti-U1 RNA antibodies in patients with connective tissue disease. Association with HLA and clinical manifestations of disease

Arthritis Rheum. 1995 Dec;38(12):1837-44. doi: 10.1002/art.1780381218.


Objective: To determine the prevalence of anti-U1 RNA antibodies in connective tissue disease (CTD) patients and evaluate immunogenetic and clinical features of patients possessing these antibodies.

Methods: RNA immunoprecipitation was used to analyze patient and healthy control sera for the presence of anti-R1 RNA antibodies. Enzyme-linked immunosorbent assay and immunoblotting were used to determine small nuclear RNP (snRNP) polypeptide antibodies. HLA polymorphisms were determined by microcytotoxicity and DNA typing.

Results: Anti-U1 RNA IgM and IgG antibodies were found in 60% of anti-RNP positive patients. All of the anti-U1 RNA positive patients had anti-70K, and most had anti-A, (U1)snRNP polypeptide antibodies. HLA-DR2/DR4, as well as Raynaud's phenomenon and synovitis, were significantly increased in the anti-U1 RNA positive group.

Conclusion: The presence of anti-U1 RNA antibodies correlates with anti-70K and anti-A polypeptide antibodies. In addition, the anti-U1 RNA positive CTD patient group is immunogenetically and clinically distinctive from the anti-U1 RNA negative patient group.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Antinuclear / metabolism
  • Autoantibodies / metabolism*
  • Child
  • Connective Tissue Diseases / complications
  • Connective Tissue Diseases / genetics
  • Connective Tissue Diseases / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • HLA Antigens / metabolism*
  • HLA-DR2 Antigen / metabolism
  • HLA-DR4 Antigen / metabolism
  • Humans
  • Immunoglobulin G / metabolism
  • Immunoglobulin M / metabolism
  • Male
  • Middle Aged
  • Precipitin Tests
  • RNA / immunology*
  • Raynaud Disease / etiology
  • Ribonucleoprotein, U1 Small Nuclear / genetics*
  • Ribonucleoprotein, U1 Small Nuclear / immunology
  • Synovitis / etiology


  • Antibodies, Antinuclear
  • Autoantibodies
  • HLA Antigens
  • HLA-DR2 Antigen
  • HLA-DR4 Antigen
  • Immunoglobulin G
  • Immunoglobulin M
  • Ribonucleoprotein, U1 Small Nuclear
  • RNA