Expression of photoreceptor cyclic nucleotide-gated cation channel alpha subunit (CNGCalpha) in the liver and skeletal muscle

FEBS Lett. 1996 Oct 14;395(1):77-81. doi: 10.1016/0014-5793(96)01011-3.

Abstract

Glucagon and beta-adrenergic agents increase cAMP levels and stimulate Ca2+ influx in liver cells. There is no consensus as to the mechanism by which these hormones stimulate the influx of Ca2+. Using mouse retinal rod CNGCalpha cDNA probes, we cloned rat liver and skeletal muscle, and human hepatic CNGCalpha subunit sequences showing 97-100% identity with the human rod channel. In order to assess channel activity, the effect of cyclic nucleotides on free intracellular Ca2+ levels of isolated hepatocytes was measured. Dibutyryl-cAMP was more effective in increasing free Ca2+ levels than dibutyryl-cGMP. These data indicate that the CNGCalpha subunit is expressed in both the liver and skeletal muscle possibly mediating hormonal effects on ion fluxes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bucladesine / pharmacology
  • Calcium / metabolism
  • Cell Membrane / chemistry
  • Cyclic Nucleotide-Gated Cation Channels
  • Dibutyryl Cyclic GMP / pharmacology
  • Gene Expression
  • Humans
  • Ion Channel Gating / drug effects*
  • Ion Channels / analysis
  • Ion Channels / chemistry
  • Ion Channels / genetics*
  • Liver / chemistry*
  • Liver / physiology
  • Male
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Muscle, Skeletal / chemistry*
  • Muscle, Skeletal / physiology
  • Nitroprusside / pharmacology
  • Nucleotides, Cyclic / pharmacology*
  • RNA, Messenger / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Retinal Rod Photoreceptor Cells / physiology
  • Sequence Homology, Amino Acid

Substances

  • Cyclic Nucleotide-Gated Cation Channels
  • Ion Channels
  • Nucleotides, Cyclic
  • RNA, Messenger
  • Nitroprusside
  • Dibutyryl Cyclic GMP
  • Bucladesine
  • Calcium