Site-directed mutagenesis using overlap extension PCR

Methods Mol Biol. 1996;57:177-91. doi: 10.1385/0-89603-332-5:177.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA Primers / genetics
  • DNA Restriction Enzymes
  • DNA, Recombinant / genetics*
  • Escherichia coli / genetics
  • Genetic Vectors / genetics
  • Mutagenesis, Site-Directed*
  • Nucleic Acid Denaturation
  • Polymerase Chain Reaction / methods*
  • Templates, Genetic

Substances

  • DNA Primers
  • DNA, Recombinant
  • DNA Restriction Enzymes