Induction of muscarinic receptor subtypes in monocytic/macrophagic cells differentiated from EoL-1 cells

Eur J Pharmacol. 1996 Feb 15;297(1-2):121-7. doi: 10.1016/0014-2999(95)00722-9.


We demonstrated that eosinophilic leukemia cell line-1 (EoL-1 cells) differentiated into monocytic/macrophagic cells by a treatment with interferon-gamma. Muscarinic receptor mRNA was not detected in untreated EoL-1 cells, and the cytosolic concentration of Ca2+ ([Ca2+]i) did not rise either in these cells. Interestingly, when EoL-1 cells were treated with interferon-gamma, mRNAs for muscarinic M3 and M5 receptors could be detected in these cells, along with an increase in [Ca2+]i and chemotaxis induced by carbachol that could be blocked with 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. These data support the functional importance of muscarinic M3 and M5 receptors in monocytic/macrophagic cells differentiated from EoL-1 cells. This model also provides evidence of a significant functional interaction between muscarinic M3 and M5 receptors.

MeSH terms

  • Calcium / metabolism
  • Carbachol / antagonists & inhibitors
  • Carbachol / pharmacology
  • Cell Differentiation
  • Chemotaxis, Leukocyte / drug effects
  • Humans
  • Interferon-gamma / pharmacology
  • Macrophages / metabolism*
  • Monocytes / metabolism*
  • Muscarinic Agonists / pharmacology
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Receptors, Muscarinic / biosynthesis
  • Receptors, Muscarinic / drug effects
  • Receptors, Muscarinic / physiology*
  • Recombinant Proteins
  • Tumor Cells, Cultured


  • Muscarinic Agonists
  • RNA, Messenger
  • Receptors, Muscarinic
  • Recombinant Proteins
  • Interferon-gamma
  • Carbachol
  • Calcium