Abstract
An electrophysiological preparation of Ascaris suum pharyngeal muscle suitable for recording changes of input conductance using a 2-microelectrode current clamp and pharmacological study is described. The preparation is shown to contain a glutamate-gated Cl (ion sensitive) channel sensitive to the avermectin analogue, milbemycin D. The application of glutamate produces a dose-dependent increase in Cl conductance and the effect of glutamate is potentiated by milbemycin D. Milbemycin D also produced a dose-dependent increase in input conductance.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Anthelmintics / pharmacology*
-
Anti-Bacterial Agents
-
Ascaris suum / physiology*
-
Chloride Channels / physiology
-
Chlorides / pharmacology
-
Drug Synergism
-
Electric Conductivity
-
Glutamic Acid / pharmacology*
-
Ion Channel Gating / drug effects*
-
Ivermectin / analogs & derivatives
-
Macrolides / pharmacology
-
Membrane Potentials
-
Pharyngeal Muscles / physiology
-
Receptors, Glutamate / physiology
Substances
-
Anthelmintics
-
Anti-Bacterial Agents
-
Chloride Channels
-
Chlorides
-
Macrolides
-
Receptors, Glutamate
-
Glutamic Acid
-
Ivermectin
-
moxidectin