Sympathetic-dependence in bradykinin-induced synovial plasma extravasation is dose-related

Neurosci Lett. 1996 Mar 1;205(3):165-8. doi: 10.1016/0304-3940(96)12403-4.


While previous studies have implicated a role for sympathetic postganglionic neuron-terminals in bradykinin-induced plasma extravasation, a recent report by Cambridge and Brain [Br. J. Pharmacol., 115 (1995) 641-647] has suggested that it is sympathetic-independent. However, the doses of bradykinin used in these two groups of studies were considerably different. Therefore, in the present study, we characterized the sympathetic-dependence of plasma extravasation at varying doses of bradykinin. By measuring the concentration of Evans blue dye extravasation into the joint perfusate following its intravenous injection, bradykinin-induced plasma extravasation in the knee joint cavity was determined spectrophotometrically. To examine the role of sympathetic postganglionic neuron terminals in mediating bradykinin-induced plasma extravasation, we used surgical ablation of the lumbar sympathetic chain. Intra-articular perfusion of BK dose-dependently increased synovial plasma extravasation. After surgical sympathectomy, the dose-response curve for bradykinin-induced plasma extravasation was significantly shifted to the right. We conclude that at concentrations observed in inflamed tissues (between 10(-8) and 10(-7) M), bradykinin-induced plasma extravasation is largely mediated by sympathetic postganglionic neuron terminals.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bradykinin / pharmacology*
  • Coloring Agents / pharmacokinetics
  • Dose-Response Relationship, Drug
  • Evans Blue / pharmacokinetics
  • Extravasation of Diagnostic and Therapeutic Materials / metabolism
  • Knee Joint / immunology
  • Knee Joint / metabolism
  • Knee Joint / physiopathology
  • Male
  • Plasma / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sympathetic Fibers, Postganglionic / drug effects*
  • Sympathetic Fibers, Postganglionic / metabolism
  • Synovial Fluid / drug effects*
  • Synovial Fluid / metabolism
  • Synovitis / metabolism


  • Coloring Agents
  • Evans Blue
  • Bradykinin