Effect of pyridone carboxylic acid anti-microbials on the generation of reactive oxygen species in vitro

J Int Med Res. Jul-Aug 1996;24(4):345-51. doi: 10.1177/030006059602400405.

Abstract

The effects of ofloxacin, ciprofloxacin and balofloxacin on the reactive oxygen species (ROS) levels generated by human neutrophils was examined in vitro; ROS generated in a cell-free, xanthine-xanthine oxidase system was also assessed. The species investigated were superoxide radical anion (O2-), hydrogen peroxide (H2O2) and hydroxyl radical (OH*). Both ofloxacin and ciprofloxacin markedly decreased the levels of O2-, H2O2 and OH* generated by human neutrophils. On the other hand, these drugs did not affect any of the ROS examined in the xanthine-xanthine oxidase system. Balofloxacin showed no significant effect on ROS generated by either system. The present study indicates that ofloxacin and ciprofloxacin may exert an anti-inflammatory action by reducing the potent ROS species excessively generated by neutrophils at the sites of inflammation.

MeSH terms

  • Anti-Infective Agents*
  • Ciprofloxacin / pharmacology*
  • Fluoroquinolones*
  • Free Radicals
  • Humans
  • Hydrogen Peroxide / metabolism
  • Neutrophils / drug effects
  • Ofloxacin / pharmacology*
  • Quinolones / pharmacology*
  • Reactive Oxygen Species / metabolism*
  • Xanthine Oxidase / drug effects
  • Xanthine Oxidase / metabolism

Substances

  • Anti-Infective Agents
  • Fluoroquinolones
  • Free Radicals
  • Quinolones
  • Reactive Oxygen Species
  • Ciprofloxacin
  • Ofloxacin
  • Hydrogen Peroxide
  • Xanthine Oxidase
  • balofloxacin