Parallel human genome analysis: microarray-based expression monitoring of 1000 genes

Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10614-9. doi: 10.1073/pnas.93.20.10614.


Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • DNA, Complementary / genetics*
  • Gene Expression* / drug effects
  • Gene Library*
  • Heat-Shock Response*
  • Humans
  • Microchemistry
  • Nucleic Acid Hybridization / methods*
  • RNA, Messenger / genetics
  • T-Lymphocytes / physiology*
  • Tetradecanoylphorbol Acetate / pharmacology*


  • DNA, Complementary
  • RNA, Messenger
  • Tetradecanoylphorbol Acetate