Abstract
The c-Myc oncoprotein has previously been shown to associate with transcription regulator YY1 and to inhibit its activity. We show herein that endogenous c-Myc and YY1 associate in vivo and that changes in c-Myc levels, which accompany mitogenic stimulation or differentiation of cultured cells, affect the ratio of free to c-Myc-associated YY1. We have also investigated the mechanism by which association with c-Myc inhibits YY1's ability to regulate transcription. c-Myc does not block binding of YY1 to DNA. However, protein association studies suggest that c-Myc interferes with the ability of YY1 to contact basal transcription proteins TATA-binding protein and TFIIB.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Binding, Competitive
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DNA / metabolism
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DNA-Binding Proteins / metabolism*
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Erythroid-Specific DNA-Binding Factors
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Gene Expression Regulation
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Mice
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Precipitin Tests
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Protein Binding
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Proto-Oncogene Proteins c-myc / metabolism*
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Recombinant Proteins
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TATA-Box Binding Protein
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Transcription Factor TFIIB
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Transcription Factors / metabolism*
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YY1 Transcription Factor
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Zinc Fingers
Substances
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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Proto-Oncogene Proteins c-myc
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Recombinant Proteins
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TATA-Box Binding Protein
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Transcription Factor TFIIB
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Transcription Factors
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YY1 Transcription Factor
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Yy1 protein, mouse
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DNA