Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids

Anim Genet. 1996 Aug;27(4):275-8. doi: 10.1111/j.1365-2052.1996.tb00489.x.

Abstract

Degenerate primers based on human and mouse obesity gene (OBS) sequencing data were used in the reverse transcriptase-polymerase chain reaction (RT-PCR) of total RNA from pig white adipose tissue. Both strands of the resultant pig- specific 325 bp DNA fragment were sequenced. Comparison of the obtained sequence with known sequences revealed an 86% identity with the human and 84% identity with the mouse OBS cDNA. The OBS gene was physically mapped to pig chromosome 18 by PCR analysis of somatic cell hybrids, using pig-specific primers. This result is consistent with the recent assignment of the human OBS gene to chromosome 7 and the observation made by comparative mapping that by using a human chromosome 7 specific library two segments of conserved synteny were detected on porcine chromosomes 9 and 18. We conclude the border of conserved synteny to be in the 7q31-7q32 region of the human chromosome.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism
  • Animals
  • Base Sequence
  • Chromosome Mapping*
  • Conserved Sequence
  • DNA Primers
  • Genetic Markers
  • Humans
  • Hybrid Cells
  • Leptin
  • Mice
  • Molecular Sequence Data
  • Obesity / genetics
  • Obesity / veterinary*
  • Polymerase Chain Reaction
  • Proteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Swine
  • Swine Diseases / genetics*

Substances

  • DNA Primers
  • Genetic Markers
  • Leptin
  • Proteins

Associated data

  • GENBANK/U40812