Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Oct 17;383(6601):641-4.
doi: 10.1038/383641a0.

Retrotransposon Reverse-Transcriptase-Mediated Repair of Chromosomal Breaks

Affiliations

Retrotransposon Reverse-Transcriptase-Mediated Repair of Chromosomal Breaks

S C Teng et al. Nature. .

Abstract

The abundance of short and long interspersed nuclear sequences (SINEs and LINEs) and pseudogenes in eukaryotic genomes indicates that reverse transcriptase (RT)-mediated phenomena are important in genome evolution. However, the mechanisms involved in their spread are largely unknown. We have developed a selection system in the yeast Saccharomyces cerevisiae to test whether RT-mediated events could be linked to the repair of double-strand breaks (DSBs). Here we show that DSBs can be fixed by the insertion of complementary DNAs at the break site. In the presence of functional RT (from human L1, yeast Tyl or Crithidia CRE1), and in the absence of homologous recombination, an HO endonuclease-induced DSB at the mating type (MAT) locus is the primary site at which a marked cDNA is observed among surviving cells. The structure and junctional sequences of these insertions suggest that repair occurs primarily by non-homologous recombination. Our data support a role for endogenous retroelements in the repair of chromosomal breaks.

Comment in

Similar articles

See all similar articles

Cited by 80 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback