Identification of Neurogenin, a Vertebrate Neuronal Determination Gene

Cell. 1996 Oct 4;87(1):43-52. doi: 10.1016/s0092-8674(00)81321-5.

Abstract

Several bHLH proteins are involved in vertebrate neurogenesis, but those controlling early stages of neuronal determination have not yet been identified. Here we describe a novel, NeuroD-related bHLH protein, NEUROGENIN, whose expression precedes that of NeuroD in both mouse and Xenopus. Expression of Xenopus NEUROGENIN-related-1 (X-NGNR-1) defines the three prospective territories of primary neurogenesis. Overexpression of X-NGNR-1 (or NEUROGENIN) induces ectopic neurogenesis and ectopic expression of XNeuroD mRNA. Endogenous X-ngnr-1 expression becomes restricted to subsets of cells by lateral inhibition, mediated by X-Delta-1 and X-Notch. The properties of X-NGNR-1 are thus analogous to those of the Drosophila proneural genes, suggesting that it functions as a vertebrate neuronal determination factor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • Carrier Proteins
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Ectoderm / chemistry
  • Gene Expression Regulation, Developmental*
  • Genes, Regulator / genetics
  • Helix-Loop-Helix Motifs
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics
  • Mice
  • Molecular Sequence Data
  • Morphogenesis
  • Nerve Tissue Proteins / genetics
  • Nervous System / chemistry
  • Nervous System / embryology*
  • Neuropeptides / chemistry
  • Neuropeptides / genetics*
  • Proteins / genetics
  • RNA, Messenger / analysis
  • Receptor, Notch1
  • Receptors, Cell Surface*
  • Transcription Factors*
  • Tubulin / genetics
  • Xenopus / genetics
  • Xenopus Proteins*

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • Carrier Proteins
  • DNA, Complementary
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Neuropeptides
  • Notch1 protein, mouse
  • Proteins
  • RNA, Messenger
  • Receptor, Notch1
  • Receptors, Cell Surface
  • Transcription Factors
  • Tubulin
  • Xenopus Proteins
  • delta protein
  • neurogenin, Xenopus
  • noggin protein
  • NeuroD protein

Associated data

  • GENBANK/U67776
  • GENBANK/U67777
  • GENBANK/U67778
  • GENBANK/U67779