Human TAFII 105 is a cell type-specific TFIID subunit related to hTAFII130

Cell. 1996 Oct 4;87(1):137-46. doi: 10.1016/s0092-8674(00)81330-6.

Abstract

We previously characterized Drosophila and human TAF subunits that make up the core TFIID complex found in all cells. Here, we report that differentiated B cells contain a novel substoichiometric TAF of 105 kDa not found associated with TFIID isolated from other cell types. The cDNA encoding hTAFII105 reveals a highly conserved C-terminal domain shared by hTAFII130 and oTAFII110, while the N-terminal coactivator domain has diverged significantly. All cells tested express TAFII105 mRNA, but only B cells contain significant levels of protein associated with TFIID. Transient overexpression of hTAFII105 selectively squelches the transcription of some genes in B cells. These properties suggest that TAFII105 is a cell type-specific subunit of TFIID that may be responsible for mediating transcription by a subset of activators in B cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / chemistry*
  • B-Lymphocytes / cytology
  • Cell Differentiation
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / analysis*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism
  • Drosophila
  • Drosophila Proteins*
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • Recombinant Fusion Proteins
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • TATA-Binding Protein Associated Factors*
  • Trans-Activators / genetics
  • Transcription Factor TFIID
  • Transcription Factors / analysis*
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • Drosophila Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • TAF1C protein, human
  • TAF4 protein, human
  • TAF4B protein, human
  • TATA-Binding Protein Associated Factors
  • Taf4 protein, Drosophila
  • Trans-Activators
  • Transcription Factor TFIID
  • Transcription Factors

Associated data

  • GENBANK/Y09321