Medium weight neurofilament mRNA in goldfish Mauthner axoplasm

Neurosci Lett. 1996 Aug 2;213(2):83-6. doi: 10.1016/0304-3940(96)12860-3.


Although axons are generally considered to lack the ability to synthesize proteins, the Mauthner axon (M-axon) of the goldfish has been reported to contain some of the basic components of the translational machinery, such as transfer RNA (tRNA), ribosomal RNA (rRNA), and ribosomes. To determine if the M-axon also contains mRNA, we isolated samples of M-axoplasm free of glial contamination as demonstrated by the absence of glial-specific mRNA and protein. Reverse transcription-polymerase chain reaction (RT-PCR) of M-axoplasmic cDNA in the presence of primers for the goldfish medium-weight neurofilament (NF-M) gene produced a single product of the expected length for RT-PCR amplification of goldfish NF-M mRNA. This mRNA might direct protein synthesis of NF-M within the M-axoplasm.

MeSH terms

  • Animals
  • Axonal Transport / genetics*
  • Axons / chemistry
  • Axons / physiology
  • DNA, Complementary
  • Glial Fibrillary Acidic Protein / genetics
  • Goldfish / physiology*
  • Immunoblotting
  • Neurofilament Proteins / biosynthesis
  • Neurofilament Proteins / genetics*
  • Neurons / physiology*
  • Neurons / ultrastructure
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Silver Staining


  • DNA, Complementary
  • Glial Fibrillary Acidic Protein
  • Neurofilament Proteins
  • RNA, Messenger
  • neurofilament protein M