Expression of dystrophin Dp71 during PC12 cell differentiation

Neurosci Lett. 1996 Aug 2;213(2):107-10. doi: 10.1016/0304-3940(96)12863-9.

Abstract

The expression of dystrophin-protein 71 (Dp71) was investigated during nerve growth factor (NGF) induced differentiation of PC12 cells. A semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was designed to measure Dp71 mRNA, whereas the Dp71 protein amount was evaluated by immunoblot analysis using an anti-dystrophin monoclonal antibody. Comparison with control cultures showed that Dp71 mRNA and protein levels increased in parallel with NGF treatment peaking with increments of 60% and 1.4 times, respectively. The upregulation of Dp71 expression during PC12 cells differentiation point at PC12 cells as a suitable model for studying the function of Dp71 in neuronal cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Differentiation / physiology
  • Dystrophin / analogs & derivatives*
  • Dystrophin / genetics
  • Gene Expression / drug effects
  • Gene Expression / physiology
  • Microtubule-Associated Proteins / analysis
  • Nerve Growth Factors / pharmacology
  • PC12 Cells / cytology*
  • PC12 Cells / drug effects
  • PC12 Cells / physiology*
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Rats
  • Time Factors

Substances

  • Dystrophin
  • Microtubule-Associated Proteins
  • Nerve Growth Factors
  • RNA, Messenger
  • apo-dystrophin 1