Involvement of N-acyl-L-hormoserine lactone autoinducers in controlling the multicellular behaviour of Serratia liquefaciens

Mol Microbiol. 1996 Apr;20(1):127-36. doi: 10.1111/j.1365-2958.1996.tb02495.x.


Several bacterial species possess the ability to differentiate into highly motile swarmer cells capable of rapid surface colonization. In Serratia liquefaciens, we demonstrate that initiation of swarmer-cell differentiation involves diffusible signal molecules that are released into the growth medium. Using high-performance liquid chromatography (HPLC), high resolution mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy, we identified N-butanoyl-L-homoserine lactone (BHL) and N-hex anoyl-L-homoserine lactone (HHL) in cell-free Serratia culture supernatants. BHL and HHL are present in a ratio of approximately 10:1 and their structures were unequivocally confirmed by chemical synthesis. The swrl (swarmer initiation) gene, the predicted translation product of which exhibits substantial homology to the LuxI family of putative N-acyl homoserine lactone (AHL) synthases is responsible for directing synthesis of both BHL and HHL. In an swrl mutant, swarming motility is abolished but can be restored by the addition of an exogenous AHL. These results add swarming motility to the rapidly expanding list of phenotypes known to be controlled through quorum sensing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Butyrolactone / analogs & derivatives*
  • 4-Butyrolactone / biosynthesis
  • 4-Butyrolactone / isolation & purification
  • 4-Butyrolactone / metabolism
  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Chromatography, High Pressure Liquid
  • Culture Media
  • Genes, Bacterial
  • Molecular Sequence Data
  • Movement
  • Phenotype
  • Serratia / genetics
  • Serratia / growth & development
  • Serratia / physiology*
  • Signal Transduction


  • Bacterial Proteins
  • Culture Media
  • N-butyrylhomoserine lactone
  • N-hexanoyl-L-homoserine lactone
  • SwrI protein, Serratia liquefaciens
  • 4-Butyrolactone

Associated data

  • GENBANK/U22823