Mapping amino acids of the measles virus hemagglutinin responsible for receptor (CD46) downregulation

Virology. 1996 Oct 1;224(1):334-7. doi: 10.1006/viro.1996.0538.

Abstract

We compared the amino acid sequences of groups of receptor (CD46) downregulating and nondownregulating measles virus (MV) hemagglutinins (Hs) and identified seven group-specific differences as candidates for the mediation of the observed differential effects. Using site-directed mutagenesis, we mutated the chosen amino acids of the H of MV-strain WTF (WTF-H), a nondownregulating H, and Introduced the corresponding amino acids of Edmonston-H (Edm-H), a downregulating H. We identified four amino acids, 211G, 243R, 451V, and 481Y, which influenced the downregulative function when introduced into WTF-H. The double mutation 451V and 481Y in WTF-H led to a degree of CD46 downregulation comparable to that of Edm-H. Conversely, introducing amino acids 451E and 481N into Edm-H resulted in a loss of the downregulative function. These results indicate that these amino acids play a decisive role in the H-CD46 interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism*
  • Binding Sites
  • Down-Regulation
  • Hemagglutinins, Viral / chemistry
  • Hemagglutinins, Viral / genetics
  • Hemagglutinins, Viral / metabolism*
  • Measles virus / metabolism*
  • Membrane Cofactor Protein
  • Membrane Glycoproteins / metabolism*
  • Mutagenesis, Site-Directed
  • Receptors, Virus / metabolism*

Substances

  • Antigens, CD
  • Hemagglutinins, Viral
  • Membrane Cofactor Protein
  • Membrane Glycoproteins
  • Receptors, Virus
  • hemagglutinin protein G, measles virus