Early diagnosis of Hepatitis B by Dane particle associated DNA polymerase assay

J Gen Virol. 1977 Jul;36(1):217-20. doi: 10.1099/0022-1317-36-1-217.


We have studied prospectively 178 subjects exposed to hepatitis B and 120 haemodialysed patients for the presence of HGs antigen, e antigen and DNA polymerase as well as for anti-HBs and anti-HBc antibodies. The results suggest that the DNA polymerase assay enables us to diagnose hepatitis B earlier than the radioimmunoassay for HGs and that DNA polymerase might be present in the blood in the absence of HBs in cases of confirmed hepatitis B. A posititive correlation between e antigen and DNA polymerase was observed in 83% of the patients on haemodialysis who developed hepatitis B but only in 9% of normal patients developing the same disease.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Viral / analysis
  • Clinical Enzyme Tests
  • DNA-Directed DNA Polymerase / blood*
  • Diagnosis, Differential
  • Evaluation Studies as Topic
  • Hepatitis B / diagnosis*
  • Hepatitis B / immunology
  • Hepatitis B Antigens / analysis*
  • Humans
  • Radioimmunoassay
  • Renal Dialysis


  • Antibodies, Viral
  • Hepatitis B Antigens
  • DNA-Directed DNA Polymerase