Characteristics of a human cell line transformed by DNA from human adenovirus type 5

J Gen Virol. 1977 Jul;36(1):59-74. doi: 10.1099/0022-1317-36-1-59.


Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides synthesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in otheruman cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 250 K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a portion of the adenovirus genome at the conventional left hand end.

MeSH terms

  • Adenoviruses, Human* / immunology
  • Adenoviruses, Human* / metabolism
  • Animals
  • Antigens, Neoplasm / analysis
  • Antigens, Viral / analysis
  • Cell Division
  • Cell Line
  • Cell Transformation, Neoplastic*
  • DNA, Viral* / biosynthesis
  • Glycoproteins / biosynthesis
  • Humans
  • Mice
  • Neoplasm Transplantation
  • Peptide Biosynthesis
  • Viral Proteins / biosynthesis


  • Antigens, Neoplasm
  • Antigens, Viral
  • DNA, Viral
  • Glycoproteins
  • Viral Proteins