Age-related assessment of central 5-HT1A receptors following irreversible inactivation by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ)

Brain Res. 1996 Jul 22;728(1):130-4. doi: 10.1016/s0006-8993(96)00481-7.


Age-dependent differences in the ability of N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) to irreversibly inactivate 5-HT1A receptors were investigated in female Fischer 344 rats (ages 3 and 22 months). In the hippocampus, frontal cortex and amygdala, EEDQ reduced 5-HT1A receptor density (33-70%) and drug affinity (2.3-6.2 fold) as determined by Scatchard analyses using [3H]8-hydroxy-2-(di-N-propylamino)tetralin. In the frontal cortex, the reduction in Bmax values was significantly greater in 3 months vs. 22 months groups. These region-specific and age-dependent alterations in 5-HT1A receptors may be of pathophysiological significance in age-related cognitive decline and Alzheimer's disease.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / metabolism*
  • Animals
  • Brain / drug effects*
  • Brain / metabolism
  • Evaluation Studies as Topic
  • Female
  • Neurotoxins / toxicity*
  • Quinolines / toxicity*
  • Rats
  • Rats, Inbred F344
  • Serotonin Antagonists / toxicity*


  • Neurotoxins
  • Quinolines
  • Serotonin Antagonists
  • EEDQ