Abstract
The affinity of rat peripheral nerve fibres for concanavalin A (Con A) and wheat germ agglutinin (WGA) was tested in semi-thin sections of Epon-embedded material. A two-step post-embedding technique was used. As a first step, Con A and WGA were used in pure form. As a second step, peroxidase-gold (for Con A) and ovomucoid-gold (for WGA) complexes were applied. The lectin-binding sites, visualized by means of signal amplification with the photochemical silver reaction, were associated mainly with the myelin sheaths and the surfaces of Schwann cells.
MeSH terms
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Animals
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Binding Sites
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Concanavalin A / metabolism*
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Epoxy Resins / chemistry
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Glycoproteins / chemistry
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Immunoenzyme Techniques
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Immunohistochemistry
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Lectins / chemistry
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Myelin Sheath / metabolism
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Nerve Fibers / metabolism*
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Ovomucin / chemistry
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Ovomucin / metabolism
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Peripheral Nervous System / metabolism
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Plastic Embedding
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Rats
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Rats, Wistar
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Schwann Cells / cytology
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Schwann Cells / metabolism
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Silver Staining
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Wheat Germ Agglutinins / metabolism*
Substances
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Epoxy Resins
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Glycoproteins
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Lectins
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Wheat Germ Agglutinins
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Concanavalin A
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Ovomucin
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EPON