Distribution of concanavalin A and wheat germ agglutinin binding sites in the rat peripheral nerve fibres revealed by lectin/glycoprotein-gold histochemistry

Histochem J. 1996 Jan;28(1):7-12. doi: 10.1007/BF02331422.

Abstract

The affinity of rat peripheral nerve fibres for concanavalin A (Con A) and wheat germ agglutinin (WGA) was tested in semi-thin sections of Epon-embedded material. A two-step post-embedding technique was used. As a first step, Con A and WGA were used in pure form. As a second step, peroxidase-gold (for Con A) and ovomucoid-gold (for WGA) complexes were applied. The lectin-binding sites, visualized by means of signal amplification with the photochemical silver reaction, were associated mainly with the myelin sheaths and the surfaces of Schwann cells.

MeSH terms

  • Animals
  • Binding Sites
  • Concanavalin A / metabolism*
  • Epoxy Resins / chemistry
  • Glycoproteins / chemistry
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Lectins / chemistry
  • Myelin Sheath / metabolism
  • Nerve Fibers / metabolism*
  • Ovomucin / chemistry
  • Ovomucin / metabolism
  • Peripheral Nervous System / metabolism
  • Plastic Embedding
  • Rats
  • Rats, Wistar
  • Schwann Cells / cytology
  • Schwann Cells / metabolism
  • Silver Staining
  • Wheat Germ Agglutinins / metabolism*

Substances

  • Epoxy Resins
  • Glycoproteins
  • Lectins
  • Wheat Germ Agglutinins
  • Concanavalin A
  • Ovomucin
  • EPON