A chemiluminescence immunoassay for evaluation of Cryptosporidium parvum growth in vitro

FEMS Microbiol Lett. 1996 Mar 1;136(3):251-6. doi: 10.1111/j.1574-6968.1996.tb08057.x.

Abstract

A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with 2 x 10(4) parasites/well. Two compounds (paromomycin and maduramicin) were evaluated and shown to have selective activity against C. parvum in a dose-dependent manner. There was excellent correlation between CLIA and immunofluorescence assay when assessing anti-C. parvum agents in MDCK cells. CLIA offers advantages over conventional enzyme-linked immunosorbent assay and immunofluorescence assay methods in that it is more sensitive and efficient. The combination of CLIA and MDCK culture provides an efficient tool for evaluating potential anti-cryptosporidial compounds prior to testing in animal models.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cell Line / parasitology
  • Cryptosporidium parvum / growth & development*
  • Dogs
  • Evaluation Studies as Topic
  • Immunoassay
  • Kidney Tubules, Distal / cytology
  • Luminescent Measurements
  • Microbiological Techniques
  • Reproducibility of Results
  • Sensitivity and Specificity