NASBA: a novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements

Clin Lab Med. 1996 Mar;16(1):89-103.


Although immunoassays have long served as the standard in the field of diagnostics, the advent of nucleic acid amplification technologies allows for a new array of diagnostic applications. NASBA, nucleic acid sequence-based amplification, is one such technology that is highly suited for the amplification of RNA. As such, NASBA is applied readily as a diagnostic tool for infectious diseases, particularly for RNA viruses, such as retroviruses. The development and application of NASBA technology as a qualitative and quantitative diagnostic system for HIV-1 are described in this article.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • HIV Infections / diagnosis
  • HIV Infections / virology*
  • HIV-1 / genetics*
  • HIV-1 / isolation & purification
  • Humans
  • Nucleic Acid Amplification Techniques*
  • Nucleic Acid Hybridization
  • RNA, Viral / isolation & purification*


  • RNA, Viral