Cloning and characterization of the murine B-cell specific transcriptional coactivator Bob1

Biol Chem Hoppe Seyler. 1996 Feb;377(2):139-45. doi: 10.1515/bchm3.1996.377.2.139.

Abstract

From a murine B-cell cDNA-library we have cloned a cDNA encoding the murine B-cell specific coactivator mBob1. The protein is the murine homologue to the recently described human coactivator Bob1 (hBob1), also referred to as OBF-1 or OCA-B. We have also characterized the genomic mBob1 clone. Analysis of its intron-exon structure has allowed identification of a C-terminal splice variant. mBob1 is B-cell restricted, and is found in all B-cell lines representing different stages of B-cell differentiation. mBob1 interacts with the octamer transcription factors Oct-1 and Oct-2 and stimulates transcription mediated by these factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / physiology
  • Base Sequence
  • Blotting, Northern
  • Cell Differentiation / physiology
  • Cell Nucleus / physiology
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA / analysis
  • DNA-Binding Proteins / metabolism
  • Gene Library
  • Host Cell Factor C1
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Rats
  • Trans-Activators / genetics*
  • Transcription Factors / metabolism
  • Tumor Cells, Cultured

Substances

  • DNA-Binding Proteins
  • HCFC1 protein, human
  • Hcfc1 protein, mouse
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • POU2AF1 protein, human
  • POU2F1 protein, human
  • POU2F2 protein, human
  • Pou2af1 protein, mouse
  • Pou2f1 protein, mouse
  • Pou2f1 protein, rat
  • Pou2f2 protein, mouse
  • Pou2f2 protein, rat
  • Trans-Activators
  • Transcription Factors
  • DNA