Abstract
From a murine B-cell cDNA-library we have cloned a cDNA encoding the murine B-cell specific coactivator mBob1. The protein is the murine homologue to the recently described human coactivator Bob1 (hBob1), also referred to as OBF-1 or OCA-B. We have also characterized the genomic mBob1 clone. Analysis of its intron-exon structure has allowed identification of a C-terminal splice variant. mBob1 is B-cell restricted, and is found in all B-cell lines representing different stages of B-cell differentiation. mBob1 interacts with the octamer transcription factors Oct-1 and Oct-2 and stimulates transcription mediated by these factors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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B-Lymphocytes / physiology
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Base Sequence
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Blotting, Northern
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Cell Differentiation / physiology
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Cell Nucleus / physiology
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Chromosome Mapping
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Cloning, Molecular
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DNA / analysis
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DNA-Binding Proteins / metabolism
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Gene Library
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Host Cell Factor C1
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Humans
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Mice
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Mice, Inbred C57BL
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Molecular Sequence Data
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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Rats
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Trans-Activators / genetics*
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Transcription Factors / metabolism
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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HCFC1 protein, human
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Hcfc1 protein, mouse
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Host Cell Factor C1
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Octamer Transcription Factor-1
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Octamer Transcription Factor-2
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POU2AF1 protein, human
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POU2F1 protein, human
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POU2F2 protein, human
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Pou2af1 protein, mouse
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Pou2f1 protein, mouse
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Pou2f1 protein, rat
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Pou2f2 protein, mouse
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Pou2f2 protein, rat
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Trans-Activators
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Transcription Factors
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DNA