Effect of biotin on glucokinase activity, mRNA expression and insulin release in cultured beta-cells

Acta Diabetol. 1996 Jul;33(2):154-8. doi: 10.1007/BF00569427.


Biotin is known to influence hepatic glucokinase (GK) expression both at a transcriptional and at a translational level. The aim of the present paper was to investigate the effect of biotin on pancreatic GK. For this purpose, RIN1046-38 cells were cultured in the presence of different biotin concentrations for different times; there-after, GK mRNA expression, GK activity and insulin release were studied. Results demonstrated that biotin has a biphasic effect on GK mRNA expression, being stimulatory after short-term treatment and inhibitory after longterm treatment. GK activity was increased after long-term treatment. Insulin release was not affected by biotin treatment. These data suggest that biotin may influence glucose metabolism also by acting directly at the level of beta-cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Biotin / pharmacology*
  • Cells, Cultured
  • DNA Primers
  • Dose-Response Relationship, Drug
  • Glucokinase / genetics*
  • Glucokinase / metabolism*
  • Insulin / metabolism*
  • Insulin Secretion
  • Islets of Langerhans / drug effects
  • Islets of Langerhans / metabolism*
  • RNA, Messenger / analysis*
  • Rats
  • Time Factors


  • DNA Primers
  • Insulin
  • RNA, Messenger
  • Biotin
  • Glucokinase