Production, Characterization and Control of a Neisseria Meningitidis Hexavalent Class 1 Outer Membrane Protein Containing Vesicle Vaccine

Vaccine. 1996 Jul;14(10):1001-8. doi: 10.1016/0264-410x(96)00020-5.

Abstract

An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5c,10;P1.12,13;P1.7h,4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various por A genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Outer Membrane Proteins / immunology*
  • Bacterial Vaccines / immunology*
  • Female
  • Humans
  • Meningitis, Meningococcal / prevention & control
  • Mice
  • Microscopy, Electron
  • Neisseria meningitidis / immunology*

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines