Antagonists of integrin alpha v beta 3 inhibit retinal neovascularization in a murine model

Lab Invest. 1996 Oct;75(4):563-73.


Integrin alpha v beta 3 is differentially expressed in angiogenic blood vessels in skin granulation tissue, and alpha v beta 3 antagonists inhibit angiogenesis in chick chorioallantoic membranes. In this study, we investigated the role of alpha v beta 3 in retinal neovascularization. There was no detectable signal for alpha v beta 3 by immunohistochemistry in normal human retina, but neovascular tissue removed from the surface of the retina of patients with diabetic retinopathy showed intense staining for alpha v beta 3 within the endothelial cells of new blood vessels. In a murine model of oxygen-induced ischemic retinopathy, there was intense staining for alpha v beta 3 in endothelial cells participating in neovascularization but no detectable staining in normal retinal blood vessels of adult mice. Synthetic peptides that bind alpha v beta 3 and perturb alpha v beta 3-mediated adhesion in vitro inhibited retinal neovascularization in the murine model when given by intraperitoneal or periocular injections. These data suggest that alpha v beta 3 antagonists may provide a useful adjunct for the treatment of retinal neovascularization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Diabetic Retinopathy / pathology
  • Disease Models, Animal
  • Endothelium, Vascular / chemistry
  • Endothelium, Vascular / cytology
  • Humans
  • Immunohistochemistry
  • Mice
  • Mice, Inbred C57BL
  • Oligopeptides / pharmacology
  • Peptides, Cyclic / pharmacology
  • Receptors, Vitronectin / antagonists & inhibitors*
  • Receptors, Vitronectin / physiology
  • Retinal Neovascularization / pathology
  • Retinal Neovascularization / prevention & control*
  • Retinal Vessels / chemistry
  • Up-Regulation


  • Oligopeptides
  • Peptides, Cyclic
  • Receptors, Vitronectin
  • XJ 735
  • XK 002