Monoclonal antibodies to HPV-6 L1 virus-like particles identify conformational and linear neutralizing epitopes on HPV-11 in addition to type-specific epitopes on HPV-6

Virology. 1996 Oct 15;224(2):477-86. doi: 10.1006/viro.1996.0554.


A set of 13 monoclonal antibodies (MAbs) was generated against HPV-6 L1 virus-like particles (VLPs), screened for reactivity to HPV-6 and HPV-11 L1 VLPs by ELISA, and tested for neutralization of HPV-11 infection. Both cross-reactive and type-specific epitopes were detected such that 4 of 13 MAbs reacted to surface conformational sites on HPV-6 L1 VLPs and the remaining 9 MAbs were cross-reactive to both HPV-6 and HPV-11 L1 VLPs. four of the 9 cross-reactive MAbs were able to neutralize HPV-11 infectivity, and 3 of 4 of these neutralizing MAbs (N-MAbs) identified shared surface conformational sites. One N-MAb therefore recognized a surface linear epitope as judged by positive binding to L1 in a Western blot assay. The neutralization status of these cross-reactive MAbs with regard to HPV-6 could not be assayed. These results demonstrated that the closely related HPV types 6 and 11 contain both type-specific and shared neutralizing epitopes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibodies, Viral / immunology*
  • Blotting, Western
  • Capsid Proteins*
  • Cross Reactions
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes, B-Lymphocyte / immunology*
  • Humans
  • Mice
  • Mice, Nude
  • Neutralization Tests
  • Oncogene Proteins, Viral / immunology*
  • Papillomaviridae / classification
  • Papillomaviridae / immunology*
  • Protein Conformation
  • Viral Proteins


  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Capsid Proteins
  • Epitopes, B-Lymphocyte
  • Oncogene Proteins, Viral
  • Viral Proteins
  • L1 protein, Human papillomavirus type 6