RT-PCR analysis of MMP-9 expression in human articular cartilage chondrocytes and synovial fluid cells

Biotech Histochem. 1996 Jul;71(4):208-13. doi: 10.3109/10520299609117161.


This report presents a method for analyzing matrix metalloproteinase-9 (92 kD gelatinase) MMP-9 mRNA expression using reverse transcription polymerase chain reaction (RT-PCR). With this method, chondrocytes isolated from small samples of osteoarthritic cartilage showed significantly elevated signal for MMP-9 mRNA compared to normal cartilage. In addition, cells of synovial fluid samples aspirated from osteoarthritic joints also exhibited MMP-9 expression using this technique. RT-PCR proved to be a sensitive method for assessing MMP-9 regulation in osteoarthritic and normal cartilage, and may provide a useful index of arthritic cartilage and synovial fluid cell metabolism when limited tissue is available.

MeSH terms

  • Cartilage, Articular / cytology
  • Cartilage, Articular / enzymology*
  • Cartilage, Articular / pathology
  • Collagenases / genetics*
  • Collagenases / metabolism
  • Humans
  • Matrix Metalloproteinase 9
  • Osteoarthritis / enzymology
  • Osteoarthritis / pathology
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sensitivity and Specificity
  • Synovial Fluid / cytology
  • Synovial Fluid / enzymology*


  • RNA, Messenger
  • Collagenases
  • Matrix Metalloproteinase 9