Identification of a DinB/UmuC homolog in the archeon Sulfolobus solfataricus

Mutat Res. 1996 Oct 25;357(1-2):245-53. doi: 10.1016/0027-5107(96)00164-9.


To date, eight closely related homologs of the Escherichia coli UmuC protein have been identified. All of these homologs appear to play critical roles in damage-inducible mutagenesis in enterobacteriaceae. Recently, a distantly related UmuC-homolog, DinB, has also been identified in E. coli. Using the polymerase chain reaction together with degenerate primers designed against conserved regions found in UmuC-like proteins, we have identified a new member of the UmuC-superfamily in the archeon Sulfolobus solfataricus. This new homolog shows high sequence similarity to DinB and a lower level of similarity to UmuC. As a consequence, we have called this new gene dbh (dinB homolog). Analysis of approximately 2.7 kb DNA encompassing the dbh region revealed several open reading frames (orfs). One, encoding a putative ribokinase, was located immediately upstream of dbh. This orf overlaps the dbh gene by 4 bp suggesting that both proteins might be coordinately expressed. Further upstream of the ribokinase-dbh locus was another orf encoding a potential ATPase homologous to two uncharacterized S. cerevisiae proteins (YD9346.02c and SC38KCXVI_20) and another E. coli DNA repair protein, RuvB. While this is the first report of a UmuC-like homolog in an archeon, we detected additional homologs using protein sequence comparisons in Gram-positive bacteria, cyanobacteria, and among potential human EST products, indicating that UmuC-related proteins comprise a ubiquitous superfamily of proteins probably involved in DNA repair and mutagenesis.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Amino Acid Sequence
  • Archaeal Proteins*
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Consensus Sequence
  • DNA Damage
  • DNA-Directed DNA Polymerase*
  • Escherichia coli Proteins*
  • Genes, Bacterial*
  • Humans
  • Molecular Sequence Data
  • Mutagenesis*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phylogeny
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Sulfolobus / genetics*


  • Archaeal Proteins
  • Bacterial Proteins
  • DinB protein, E coli
  • Escherichia coli Proteins
  • UmuC protein, E coli
  • Phosphotransferases (Alcohol Group Acceptor)
  • ribokinase
  • Dbh protein, Sulfolobus solfataricus
  • DNA-Directed DNA Polymerase
  • Adenosine Triphosphatases

Associated data

  • GENBANK/U52110