Mouse IL-17: a cytokine preferentially expressed by alpha beta TCR + CD4-CD8-T cells

J Interferon Cytokine Res. 1996 Aug;16(8):611-7. doi: 10.1089/jir.1996.16.611.


A novel cytokine originally designated murine CTLA-8 was described as a cDNA isolated from an activated T cell hybridoma produced by fusing a mouse cytotoxic T cell clone and a rat T lymphoma. This cDNA, which contains mRNA instability sequences characteristic of many cytokines, encoded a putative secreted protein that was homologous to the ORF13 gene of Herpesvirus saimiri. The human homolog to this molecule has recently been identified as the proinflammatory cytokine IL-17. We describe the isolation of a cDNA encoding mouse IL-17 from a cDNA library generated from alpha beta TCR + CD4-CD8- thymocytes using a subtraction technique that enriched for activation specific genes. This cDNA shares 87.3% amino acid identity to the previously described murine CTLA-8. Comparison of murine CTLA-8 to a cDNA we isolated from activated rat splenocytes revealed that murine CTLA-8 is, in fact, the rat homolog of IL-17. Mouse IL-17 mRNA is specifically expressed by activated alpha beta TCR + CD4-CD8- T cells, a small subset with a potentially important role in immune regulation. Mouse, rat, and human IL-17 can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells
  • Connective Tissue / drug effects
  • Connective Tissue / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Gene Library
  • Genes
  • Humans
  • Interleukin-17
  • Interleukin-6 / metabolism
  • Interleukins / biosynthesis
  • Interleukins / genetics*
  • Interleukins / pharmacology
  • Mice / genetics*
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Rats
  • Receptors, Antigen, T-Cell, alpha-beta / analysis*
  • Recombinant Fusion Proteins / pharmacology
  • Sequence Alignment
  • Sequence Homology
  • Species Specificity
  • Subtraction Technique
  • T-Lymphocyte Subsets / metabolism*
  • Tumor Cells, Cultured


  • DNA, Complementary
  • Interleukin-17
  • Interleukin-6
  • Interleukins
  • Receptors, Antigen, T-Cell, alpha-beta
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/U43088