Glaucoma, capillaries and pericytes. 4. Beta-adrenergic activation of cultured retinal pericytes

Ophthalmologica. 1996;210(5):276-9. doi: 10.1159/000310725.


To characterize the relaxation of pericytes induced by beta-adrenergic stimulation, changes in the contractile tone of pericytes were quantified as a change in the wrinkling of an elastic silicone surface on which they were cultured. Isoproterenol produced relaxation of pericytes in a dose-dependent manner over a range of 5 nM to 1 microM. Low concentrations of the nonselective beta-blockers propanolol and timolol blocked the relaxation produced by isoproterenol. The specific beta 2-adrenergic component of isoproterenol-induced relaxation was shown by blockage with bromoacetyl alprenolol menthane. In contrast, atenolol and betaxolol, as relatively selective beta 1-adrenergic blockers, had no effect on the isoproterenol-induced relaxation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adrenergic beta-Agonists / pharmacology
  • Adrenergic beta-Antagonists / pharmacology
  • Animals
  • Blood Flow Velocity / drug effects
  • Capillaries / cytology
  • Capillaries / metabolism*
  • Cattle
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Glaucoma / metabolism*
  • Isoproterenol / pharmacology
  • Muscle Contraction / drug effects
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism
  • Optic Nerve / blood supply
  • Receptors, Adrenergic, beta / drug effects
  • Receptors, Adrenergic, beta / metabolism*
  • Retinal Vessels / cytology
  • Retinal Vessels / metabolism*


  • Adrenergic beta-Agonists
  • Adrenergic beta-Antagonists
  • Receptors, Adrenergic, beta
  • Isoproterenol