Production of Recombinant SERA Proteins of Plasmodium Falciparum in Escherichia Coli by Using Synthetic Genes

Vaccine. 1996 Aug;14(11):1069-76. doi: 10.1016/0264-410x(95)00238-v.

Abstract

We expressed two regions of the serine repeat antigen (SERA) protein of Plasmodium falciparum in Escherichia coli by synthesizing the genes with a changed codon usage. One of the synthetic gene sequences encodes amino acid residues 17-382 (SE47') and the other encodes amino acid residues 586-802 (SE50A). The products produced by the synthetic gene sequences in E. coli accounted for 15-30% of the total bacterial protein. Antisera against both the purified gene products prepared in rats inhibited malaria parasite growth in vitro. The anti-SE47' serum was significantly more inhibitory than the anti-SE50A serum. The described methods provide a large scale preparation of recombinant antigens for improving and producing malaria vaccine.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Protozoan / immunology
  • Antigens, Protozoan / biosynthesis
  • Antigens, Protozoan / genetics*
  • Base Sequence
  • Escherichia coli / genetics*
  • Escherichia coli / immunology
  • Escherichia coli / metabolism
  • Female
  • Genes, Protozoan / immunology*
  • Genes, Synthetic / immunology*
  • Genetic Vectors / immunology
  • Malaria, Falciparum / prevention & control
  • Molecular Sequence Data
  • Plasmodium falciparum / genetics
  • Plasmodium falciparum / immunology
  • Plasmodium falciparum / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Proteins / biosynthesis*

Substances

  • Antibodies, Protozoan
  • Antigens, Protozoan
  • Recombinant Proteins
  • serine repeat antigen, Plasmodium