Neurogenesis in the adult rat: electron microscopic analysis of light radioautographs

Science. 1977 Sep 9;197(4308):1092-4. doi: 10.1126/science.887941.

Abstract

Three-month-old rats were injected intraperitoneally with [3H]thymidine (4.3 microcuries per gram of body weight) and allowed to survive for 30 days. Radioautography of 1-micrometer sections revealed labeled cells in the granular layers of dentate gyrus and olfactory bulb; these were confirmed as neurons by electron microscopy of reembedded 1-micrometer sections.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Age Factors
  • Animals
  • Hippocampus / cytology
  • Hippocampus / growth & development*
  • Hippocampus / metabolism
  • Male
  • Neurons / metabolism
  • Neurons / physiology*
  • Olfactory Bulb / cytology
  • Olfactory Bulb / growth & development*
  • Olfactory Bulb / metabolism
  • Rats
  • Thymidine / metabolism

Substances

  • Thymidine