Synthetic peptides non-covalently bound to bacterial hsp 70 elicit peptide-specific T-cell responses in vivo

Immunology. 1996 Aug;88(4):487-92. doi: 10.1046/j.1365-2567.1996.d01-697.x.

Abstract

We have examined the immunogenicity of complexes formed by non-covalent association of a synthetic peptide corresponding to influenza A virus nucleoprotein, residues 206-229 (pNP) and Mycobacterium tuberculosis heat-shock protein 70 (hsp 70). One or two injections of these complexes given to BALB/c mice without any additional adjuvant, were capable of eliciting very strong peptide-specific proliferative T-cell responses in the spleen. These responses were dependent on the stability of the complex since immunogenicity was lost when dissociated with ATP prior to immunization. T-cell responses to hsp 70 were easily generated by immunization with the purified chaperone alone, either after primary or secondary immunization. Injection of pNP-hsp 70 complexes, however, although generating good primary responses, resulted in very much decreased proliferative responses to the hsp 70.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / pharmacology
  • Amino Acid Sequence
  • Animals
  • Antigens, Bacterial / immunology*
  • Cell Division / drug effects
  • Cell Division / immunology
  • Electrophoresis, Polyacrylamide Gel
  • HSP70 Heat-Shock Proteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Mycobacterium tuberculosis / immunology*
  • Nucleocapsid Proteins
  • Nucleoproteins / immunology*
  • RNA-Binding Proteins*
  • Spleen / immunology
  • T-Lymphocytes / immunology*
  • Vaccines, Synthetic / immunology
  • Viral Core Proteins / immunology*

Substances

  • Antigens, Bacterial
  • HSP70 Heat-Shock Proteins
  • NP protein, Influenza A virus
  • Nucleocapsid Proteins
  • Nucleoproteins
  • RNA-Binding Proteins
  • Vaccines, Synthetic
  • Viral Core Proteins
  • Adenosine Triphosphate