Differences in environmental temperature, ethanol and sucrose associated with enzyme activity and weight changes in Drosophila melanogaster

Insect Biochem Mol Biol. 1996 Feb;26(2):135-45. doi: 10.1016/0965-1748(95)00072-0.


Activity changes of three enzymes (ADH, ODH and AOX) of Drosophila melanogaster were followed under different environmental conditions. The influences of ethanol, starvation (no carbohydrates in the medium) and ethanol stress during starvation were studied at both 18 and 26 degrees C. Two strains that were monomorphic for different alleles at the Odh and Aldox loci but otherwise identical were used. The investigated environmental conditions affected ADH induction by exogenous ethanol differently in the two strains. The different allozymes of ODH and AOX also responded differently to the treatments. We observed that the sucrose content of the medium on which ethanol exposure took place and the temperature strongly affected the responses within any single strain. Correlations were estimated among the three enzymes in the larval and adult stages of each strain separately. At both temperatures, differences between strains were observed in the patterns of associations of the response variables, in the larval, but not in the adult stages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase / metabolism*
  • Alcohol Oxidoreductases / metabolism*
  • Aldehyde Oxidase
  • Aldehyde Oxidoreductases / metabolism*
  • Animals
  • Body Weight
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / growth & development
  • Environment
  • Ethanol / pharmacology*
  • Female
  • Larva
  • Sucrose / pharmacology*
  • Temperature


  • Ethanol
  • Sucrose
  • Alcohol Oxidoreductases
  • Alcohol Dehydrogenase
  • octanol dehydrogenase
  • Aldehyde Oxidoreductases
  • Aldehyde Oxidase