Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: a novel cDNA family encodes cell surface proteins sharing partial homology with MHC class I molecules

J Biochem. 1996 Feb;119(2):319-28. doi: 10.1093/oxfordjournals.jbchem.a021242.


Rae-1 cDNA is one of the retinoic acid (RA) inducible cDNA clones in mouse embryonal carcinoma F9 cells. Rae-1 mRNAs were detected in mouse early embryos, but not in various tissues of adult mice. RAE-1 protein apparently consists of 253 amino acids and is likely to be a glycoprotein consisting of a leader sequence, an extracellular domain, a serine, threonine, proline-rich domain, and a transmembrane domain. Interestingly, it has a weak, but significant homology with major histocompatibility complex (MHC) class I molecules and was immunocytochemically identified as a cell surface protein. By determining partial nucleotide sequences of 17 Rae-1 cDNAs isolated from the RA-induced F9 cells, at least three different kinds of Rae-1 cDNAs were identified and were named Rae-1 alpha, Rae-1 beta, and Rae-1 gamma cDNAs, respectively. As the overall nucleotide sequence homology among these three cDNAs was about 98%, they constitute a novel gene family which is likely to be involved in early mammalian embryogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cell Line
  • Cloning, Molecular
  • DNA, Complementary
  • Histocompatibility Antigens Class I / genetics*
  • Membrane Proteins / genetics*
  • Membrane Proteins / metabolism
  • Mice
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Sequence Homology, Amino Acid
  • Tretinoin / pharmacology*


  • DNA, Complementary
  • Histocompatibility Antigens Class I
  • Membrane Proteins
  • RNA, Messenger
  • Raet1a protein, mouse
  • Raet1b protein, mouse
  • Raet1c protein, mouse
  • Tretinoin

Associated data

  • GENBANK/D64160
  • GENBANK/D64161
  • GENBANK/D64162