Lactate dehydrogenase activity in periodontal ligament cells stored in different transport media

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Sep;82(3):321-3. doi: 10.1016/s1079-2104(96)80360-x.

Abstract

Background: Maintaining viability of periodontal ligament cells is important after an avulsion injury occurs.

Objective: This study examined cell death and compared different transport media at varying times. Lactate dehydrogenase (LD) measurements were made on root surfaces of extracted teeth for evaluating the results of breakdown and necrosis of periodontal ligament cells.

Study design: Teeth were divided into three groups. Each was immersed in one of the following storage media: Hanks balanced salt solution, Custodiol solution (an organ storage medium), or sterile saline solution. I.D. measurements (an indicator of cell death) were made at 2, 6, 24, 72, and 168 hours after extraction.

Results: When the LD levels were compared according to the time periods, a progressive increase of cell death was found to be statistically significant (p < 0.001). However, when the effects of both time periods and the kind of storage medium on the LD levels were examined, the LD levels of the saline solution storage medium were found to be significantly higher (p < 0.001) than that of the other two groups.

Conclusions: Both Hanks balanced salt solution and Custodiol appeared to be suitable transport media for maintaining cell viability, whereas saline solution was not.

Publication types

  • Clinical Trial
  • Comparative Study
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Cell Death
  • Cell Survival
  • Child
  • Glucose
  • Humans
  • Isotonic Solutions
  • L-Lactate Dehydrogenase / metabolism
  • Mannitol
  • Periodontal Ligament / cytology*
  • Periodontal Ligament / enzymology
  • Potassium Chloride
  • Procaine
  • Random Allocation
  • Sodium Chloride
  • Tissue Preservation / methods*

Substances

  • Bretschneider cardioplegic solution
  • Hanks Balanced Salt Solution
  • Isotonic Solutions
  • Mannitol
  • Sodium Chloride
  • Procaine
  • Potassium Chloride
  • L-Lactate Dehydrogenase
  • Glucose