The SCID but not the RAG-2 gene product is required for S mu-S epsilon heavy chain class switching

Immunity. 1996 Oct;5(4):319-30. doi: 10.1016/s1074-7613(00)80258-7.


We have investigated the capacity of precursor B cells from normal (BDF1) and V(D)J recombinase-deficient (RAG-27) or defective (SCID) mice to be induced by a CD40-specific monoclonal antibody and IL-4 to epsilon H chain gene transcription and to S mu-S epsilon switch recombination. In differentiating precursor B cells from all three strains of mice, the development of similar numbers of CD19+, CD23+, CD40+, and MHC class II+ expressing B lineage cells and similar levels of epsilon H chain gene transcription were induced. Efficient S mu-S epsilon switching occurred in normal and RAG-2-deficient, but not in SCID, precursor B cells. Thus, the transcription of the epsilon H chain is independent of the RAG-2 and the SCID gene product, while the S mu-S epsilon switch recombination requires the SCID gene-encoded DNA-dependent protein kinase, but not the RAG-2 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • B-Lymphocytes / cytology*
  • CD40 Antigens / physiology
  • Cell Differentiation
  • DNA-Binding Proteins*
  • Gene Expression Regulation, Developmental
  • Genes, Immunoglobulin*
  • Hybridomas
  • Immunoglobulin epsilon-Chains / genetics*
  • Immunoglobulin mu-Chains / genetics*
  • Immunophenotyping
  • Interleukin-4 / pharmacology
  • Interleukin-7 / physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, SCID / immunology*
  • Proteins / physiology*
  • Recombination, Genetic


  • Antibodies, Monoclonal
  • CD40 Antigens
  • DNA-Binding Proteins
  • Immunoglobulin epsilon-Chains
  • Immunoglobulin mu-Chains
  • Interleukin-7
  • Proteins
  • Rag2 protein, mouse
  • V(D)J recombination activating protein 2
  • Interleukin-4