The aim of this study is to provide accurate three-dimensional measurements of left ventricular geometrical indices in relation to regional myocardial function. The analysis of the three-dimensional regional geometry and function of left ventricles of ten normal human volunteers is based on three-dimensional reconstructions of the left ventricle from cine computed tomography images, at end diastole and end systole, demonstrating normal left ventricular spatial, geometrical, and functional variability. Regional wall thickness, curvature and surface normals, as well as wall thickening and endocardial wall motion, are calculated and mapped for the entire left ventricle. The circumferential asymmetry of the left ventricle is reflected by the smaller circumferential and meridional curvatures at the septum. Thickening is highest at the anterior and lateral walls. Longitudinally, circumferential curvature increases toward the apex, whereas both wall thickness and wall thickening at end systole are largest at the midventricular level, decreasing toward the apex and base. This study describes the circumferential and apex-to-base variations in regional left ventricular geometric parameters of the normal human left ventricle, using three-dimensional imaging and analysis.