Abstract
Expression of HSP-27, HSP-60 and HSP-70 was estimated in the cortex, outer medulla and inner medulla (papilla) of rats undergoing water diuresis or water restriction for two days. The mRNAs for HSP-27 and HSP-60 in renal papilla were two- to threefold greater in rats during water restriction than in those excreting a dilute urine, but levels of mRNA for HSP-70 were not reduced by water diuresis and Western analysis for HSP-70 protein showed no difference between water-loaded and water-restricted animals.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Blotting, Northern
-
Blotting, Western
-
Chaperonin 60 / genetics
-
Chaperonin 60 / metabolism*
-
Diuresis / physiology*
-
HSP70 Heat-Shock Proteins / genetics
-
HSP70 Heat-Shock Proteins / metabolism*
-
Heat-Shock Proteins / genetics
-
Heat-Shock Proteins / metabolism*
-
Kidney / drug effects
-
Kidney / metabolism*
-
Kidney Cortex / physiology
-
Kidney Medulla / physiology
-
Male
-
RNA, Messenger / analysis
-
Rats
-
Rats, Sprague-Dawley
-
Water Deprivation / physiology*
Substances
-
Chaperonin 60
-
HSP70 Heat-Shock Proteins
-
Heat-Shock Proteins
-
RNA, Messenger