Alternate functions of the single-minded and rhomboid genes in development of the Drosophila ventral neuroectoderm

Mech Dev. 1996 Aug;58(1-2):65-74. doi: 10.1016/s0925-4773(96)00559-x.

Abstract

We have investigated the roles of the single-minded (sim) and rhomboid (rho) genes in generating distinct cell fates in the Drosophila embryonic neuroectoderm. We show that sim functions to repress ventral ectodermal cell fates, as in sim mutants mesectodermal cells adopt the fates of neighboring ventral ectodermal cells and targeted sim expression in P[paired.Gal4]/P[UAS-sim] embryos results in loss of epidermal cells. We also find that rho is not required for early expression of sim or ventral nervous system defective in mesectodermal or ventral ectodermal cells; targeted rho expression in P[paired-Gal4]/P[UAS-rho] embryos results in lateral-to-ventral cell fate shifts in the developing neuroectoderm; and midline-targeted rho expression can rescue the medial denticle fusions in rho mutant cuticles.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Drosophila
  • Drosophila Proteins*
  • Ectoderm / chemistry
  • Embryo, Nonmammalian / chemistry
  • Gene Expression Regulation, Developmental
  • Hedgehog Proteins
  • Helix-Loop-Helix Motifs / genetics
  • Helix-Loop-Helix Motifs / physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Insect Hormones / genetics
  • Insect Hormones / physiology
  • Insect Proteins / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Mutation
  • Nuclear Proteins / genetics
  • Nuclear Proteins / physiology*
  • Phenotype
  • Proto-Oncogene Proteins / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology
  • Wnt1 Protein

Substances

  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Drosophila Proteins
  • Hedgehog Proteins
  • Insect Hormones
  • Insect Proteins
  • Membrane Proteins
  • Nuclear Proteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Rho protein, Drosophila
  • Stet protein, Drosophila
  • Wnt1 Protein
  • sim protein, Drosophila
  • wg protein, Drosophila
  • hh protein, Drosophila