Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe

Gene. 1996 Oct 3;174(2):315-8. doi: 10.1016/0378-1119(96)00085-6.


The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cloning, Molecular
  • DNA, Recombinant
  • Gene Library*
  • Genetic Complementation Test
  • Genetic Markers
  • Genetic Variation
  • Genetic Vectors / genetics*
  • Hydro-Lyases / genetics*
  • Plasmids
  • Schizosaccharomyces / genetics*


  • DNA, Recombinant
  • Genetic Markers
  • Hydro-Lyases
  • imidazoleglycerolphosphate dehydratase