Flow cytometric identification of murine neutrophils and monocytes

J Immunol Methods. 1996 Oct 16;197(1-2):139-50. doi: 10.1016/0022-1759(96)00138-x.


Flow cytometry and cell sorting have been employed in order to identify and purify murine neutrophils and monocytes. Using a combination of antibodies, we were able to distinguish between these two subsets of myeloid cells. The method described in this paper is simple to perform and can be applied to characterize myeloid cells from blood, spleen, bone marrow and after an induced inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic / immunology*
  • B-Lymphocytes / cytology
  • Bone Marrow Cells
  • Cell Separation / methods*
  • Flow Cytometry / methods*
  • Leukocyte Common Antigens / analysis
  • Macrophage-1 Antigen / immunology
  • Macrophages, Peritoneal / cytology
  • Mice
  • Mice, Inbred C57BL
  • Monocytes / cytology*
  • Neutrophils / cytology*
  • Spleen / cytology
  • Thioglycolates


  • Antibodies, Monoclonal
  • Antigens, Differentiation, Myelomonocytic
  • Macrophage-1 Antigen
  • Thioglycolates
  • Leukocyte Common Antigens