Oncogene alterations in primary, recurrent, and metastatic human bone tumors

J Cell Biochem. 1996 Oct;63(1):37-50. doi: 10.1002/(SICI)1097-4644(199610)63:1%3C37::AID-JCB3%3E3.0.CO;2-0.


We investigated the structure and the expression of various oncogenes in three of the most common human bone tumors-osteosarcoma (36 samples from 34 patients), giant cell tumor (10 patients), and chondrosarcoma (18 patients)-in an attempt to identify the genetic alterations associated with these malignancies. Alterations of RB and p53 were detected only in osteosarcomas. Alterations of c-myc, N-myc, and c-fos were detected in osteosarcomas and giant cell tumors. Ras alterations (H-ras, Ki-ras, N-ras) were rare. Chondrosarcomas did not contain any detectable genetic alterations. Our results suggest that alterations of c-myc, N-myc, and c-fos oncogenes occur in osteosarcomas, in addition to those previously described for the tumor suppressor genes RB and p53. Moreover, statistical analyses indicate that c-fos alterations occur more frequently in osteosarcoma patients with recurrent or metastatic disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Blotting, Southern
  • Bone Neoplasms / genetics*
  • Bone Neoplasms / pathology
  • Child
  • Chondrosarcoma / genetics
  • Female
  • Genes, Tumor Suppressor
  • Genes, fos
  • Genes, myc
  • Genes, ras
  • Giant Cell Tumors / genetics
  • Humans
  • Male
  • Middle Aged
  • Oncogenes*
  • Osteosarcoma / genetics
  • Polymerase Chain Reaction
  • Tumor Suppressor Protein p53 / genetics


  • Tumor Suppressor Protein p53